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nhe3  (StressMarq)
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K ir 5.1 DN animals fail to mount an adequate physiological response to dietary K + depletion (A–C) K ir 5.1 DN mice had increased urine K + concentrations, B) urine K + excretions, and C) cumulative urine K + losses compared with K ir 5.1 WT controls on a K + -free diet. (D and E) Urine Na + D) concentrations and E) excretions did not differ between genotypes. (F–J) K ir 5.1 DN mice had increased urine Na + -to-K + ratios under K + -free conditions. After consuming a K + -free diet for four days, K ir 5.1 DN mice had G) unchanged blood Na + , H) reduced blood K + , I) unchanged blood Cl − , and J) reduced blood HCO 3 − relative to K ir 5.1 WT controls. Note, K + values in the K ir 5.1 DN group were at the lower limit of detection. A nonparametric analysis was performed for K + , given the lack of normality. (K) Urine K + excretion in two groups of K ir 5.1 DN animals. One group was treated with a K + -free diet alone, while the other group was additionally treated with the ENaC inhibitor, triamterene. (L) Kidney Western blot data after the consumption of a K + -free diet for four days. Compared with K ir 5.1 WT controls, K ir 5.1 DN mice had reduced abundances of NBCe1 and <t>NHE3</t> and increased NKCC2 abundance. Abundances of pNCC and total NCC trended toward being reduced, but did not achieve the threshold for statistical significance. Ponceau shown for pNCC and NHE3. Ponceaus for NBCe1, NKCC2, and tNCC included as . N = 5 and 7 for A-F. N = 10 and 8 for G-J. N = 4 per group for K. N = 5 per group for L. # indicates p < 0.05 by two-way ANOVA with repeated measures followed by Sidak’s post-hoc test. † indicates p < 0.05 for interaction by two-way ANOVA with repeated measures. ∗ indicates p < 0.05 by two-tailed Student’s unpaired t-test (or Mann-Whitney for H). Data are mean +/− SEM.
Nhe3, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nhe3  (Proteintech)
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K ir 5.1 DN animals fail to mount an adequate physiological response to dietary K + depletion (A–C) K ir 5.1 DN mice had increased urine K + concentrations, B) urine K + excretions, and C) cumulative urine K + losses compared with K ir 5.1 WT controls on a K + -free diet. (D and E) Urine Na + D) concentrations and E) excretions did not differ between genotypes. (F–J) K ir 5.1 DN mice had increased urine Na + -to-K + ratios under K + -free conditions. After consuming a K + -free diet for four days, K ir 5.1 DN mice had G) unchanged blood Na + , H) reduced blood K + , I) unchanged blood Cl − , and J) reduced blood HCO 3 − relative to K ir 5.1 WT controls. Note, K + values in the K ir 5.1 DN group were at the lower limit of detection. A nonparametric analysis was performed for K + , given the lack of normality. (K) Urine K + excretion in two groups of K ir 5.1 DN animals. One group was treated with a K + -free diet alone, while the other group was additionally treated with the ENaC inhibitor, triamterene. (L) Kidney Western blot data after the consumption of a K + -free diet for four days. Compared with K ir 5.1 WT controls, K ir 5.1 DN mice had reduced abundances of NBCe1 and <t>NHE3</t> and increased NKCC2 abundance. Abundances of pNCC and total NCC trended toward being reduced, but did not achieve the threshold for statistical significance. Ponceau shown for pNCC and NHE3. Ponceaus for NBCe1, NKCC2, and tNCC included as . N = 5 and 7 for A-F. N = 10 and 8 for G-J. N = 4 per group for K. N = 5 per group for L. # indicates p < 0.05 by two-way ANOVA with repeated measures followed by Sidak’s post-hoc test. † indicates p < 0.05 for interaction by two-way ANOVA with repeated measures. ∗ indicates p < 0.05 by two-tailed Student’s unpaired t-test (or Mann-Whitney for H). Data are mean +/− SEM.
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1 ap  (Proteintech)
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K ir 5.1 DN animals fail to mount an adequate physiological response to dietary K + depletion (A–C) K ir 5.1 DN mice had increased urine K + concentrations, B) urine K + excretions, and C) cumulative urine K + losses compared with K ir 5.1 WT controls on a K + -free diet. (D and E) Urine Na + D) concentrations and E) excretions did not differ between genotypes. (F–J) K ir 5.1 DN mice had increased urine Na + -to-K + ratios under K + -free conditions. After consuming a K + -free diet for four days, K ir 5.1 DN mice had G) unchanged blood Na + , H) reduced blood K + , I) unchanged blood Cl − , and J) reduced blood HCO 3 − relative to K ir 5.1 WT controls. Note, K + values in the K ir 5.1 DN group were at the lower limit of detection. A nonparametric analysis was performed for K + , given the lack of normality. (K) Urine K + excretion in two groups of K ir 5.1 DN animals. One group was treated with a K + -free diet alone, while the other group was additionally treated with the ENaC inhibitor, triamterene. (L) Kidney Western blot data after the consumption of a K + -free diet for four days. Compared with K ir 5.1 WT controls, K ir 5.1 DN mice had reduced abundances of NBCe1 and <t>NHE3</t> and increased NKCC2 abundance. Abundances of pNCC and total NCC trended toward being reduced, but did not achieve the threshold for statistical significance. Ponceau shown for pNCC and NHE3. Ponceaus for NBCe1, NKCC2, and tNCC included as . N = 5 and 7 for A-F. N = 10 and 8 for G-J. N = 4 per group for K. N = 5 per group for L. # indicates p < 0.05 by two-way ANOVA with repeated measures followed by Sidak’s post-hoc test. † indicates p < 0.05 for interaction by two-way ANOVA with repeated measures. ∗ indicates p < 0.05 by two-tailed Student’s unpaired t-test (or Mann-Whitney for H). Data are mean +/− SEM.
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K ir 5.1 DN animals fail to mount an adequate physiological response to dietary K + depletion (A–C) K ir 5.1 DN mice had increased urine K + concentrations, B) urine K + excretions, and C) cumulative urine K + losses compared with K ir 5.1 WT controls on a K + -free diet. (D and E) Urine Na + D) concentrations and E) excretions did not differ between genotypes. (F–J) K ir 5.1 DN mice had increased urine Na + -to-K + ratios under K + -free conditions. After consuming a K + -free diet for four days, K ir 5.1 DN mice had G) unchanged blood Na + , H) reduced blood K + , I) unchanged blood Cl − , and J) reduced blood HCO 3 − relative to K ir 5.1 WT controls. Note, K + values in the K ir 5.1 DN group were at the lower limit of detection. A nonparametric analysis was performed for K + , given the lack of normality. (K) Urine K + excretion in two groups of K ir 5.1 DN animals. One group was treated with a K + -free diet alone, while the other group was additionally treated with the ENaC inhibitor, triamterene. (L) Kidney Western blot data after the consumption of a K + -free diet for four days. Compared with K ir 5.1 WT controls, K ir 5.1 DN mice had reduced abundances of NBCe1 and <t>NHE3</t> and increased NKCC2 abundance. Abundances of pNCC and total NCC trended toward being reduced, but did not achieve the threshold for statistical significance. Ponceau shown for pNCC and NHE3. Ponceaus for NBCe1, NKCC2, and tNCC included as . N = 5 and 7 for A-F. N = 10 and 8 for G-J. N = 4 per group for K. N = 5 per group for L. # indicates p < 0.05 by two-way ANOVA with repeated measures followed by Sidak’s post-hoc test. † indicates p < 0.05 for interaction by two-way ANOVA with repeated measures. ∗ indicates p < 0.05 by two-tailed Student’s unpaired t-test (or Mann-Whitney for H). Data are mean +/− SEM.
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K ir 5.1 DN animals fail to mount an adequate physiological response to dietary K + depletion (A–C) K ir 5.1 DN mice had increased urine K + concentrations, B) urine K + excretions, and C) cumulative urine K + losses compared with K ir 5.1 WT controls on a K + -free diet. (D and E) Urine Na + D) concentrations and E) excretions did not differ between genotypes. (F–J) K ir 5.1 DN mice had increased urine Na + -to-K + ratios under K + -free conditions. After consuming a K + -free diet for four days, K ir 5.1 DN mice had G) unchanged blood Na + , H) reduced blood K + , I) unchanged blood Cl − , and J) reduced blood HCO 3 − relative to K ir 5.1 WT controls. Note, K + values in the K ir 5.1 DN group were at the lower limit of detection. A nonparametric analysis was performed for K + , given the lack of normality. (K) Urine K + excretion in two groups of K ir 5.1 DN animals. One group was treated with a K + -free diet alone, while the other group was additionally treated with the ENaC inhibitor, triamterene. (L) Kidney Western blot data after the consumption of a K + -free diet for four days. Compared with K ir 5.1 WT controls, K ir 5.1 DN mice had reduced abundances of NBCe1 and <t>NHE3</t> and increased NKCC2 abundance. Abundances of pNCC and total NCC trended toward being reduced, but did not achieve the threshold for statistical significance. Ponceau shown for pNCC and NHE3. Ponceaus for NBCe1, NKCC2, and tNCC included as . N = 5 and 7 for A-F. N = 10 and 8 for G-J. N = 4 per group for K. N = 5 per group for L. # indicates p < 0.05 by two-way ANOVA with repeated measures followed by Sidak’s post-hoc test. † indicates p < 0.05 for interaction by two-way ANOVA with repeated measures. ∗ indicates p < 0.05 by two-tailed Student’s unpaired t-test (or Mann-Whitney for H). Data are mean +/− SEM.
Mouse Monoclonal Anti Nhe3 Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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K ir 5.1 DN animals fail to mount an adequate physiological response to dietary K + depletion (A–C) K ir 5.1 DN mice had increased urine K + concentrations, B) urine K + excretions, and C) cumulative urine K + losses compared with K ir 5.1 WT controls on a K + -free diet. (D and E) Urine Na + D) concentrations and E) excretions did not differ between genotypes. (F–J) K ir 5.1 DN mice had increased urine Na + -to-K + ratios under K + -free conditions. After consuming a K + -free diet for four days, K ir 5.1 DN mice had G) unchanged blood Na + , H) reduced blood K + , I) unchanged blood Cl − , and J) reduced blood HCO 3 − relative to K ir 5.1 WT controls. Note, K + values in the K ir 5.1 DN group were at the lower limit of detection. A nonparametric analysis was performed for K + , given the lack of normality. (K) Urine K + excretion in two groups of K ir 5.1 DN animals. One group was treated with a K + -free diet alone, while the other group was additionally treated with the ENaC inhibitor, triamterene. (L) Kidney Western blot data after the consumption of a K + -free diet for four days. Compared with K ir 5.1 WT controls, K ir 5.1 DN mice had reduced abundances of NBCe1 and <t>NHE3</t> and increased NKCC2 abundance. Abundances of pNCC and total NCC trended toward being reduced, but did not achieve the threshold for statistical significance. Ponceau shown for pNCC and NHE3. Ponceaus for NBCe1, NKCC2, and tNCC included as . N = 5 and 7 for A-F. N = 10 and 8 for G-J. N = 4 per group for K. N = 5 per group for L. # indicates p < 0.05 by two-way ANOVA with repeated measures followed by Sidak’s post-hoc test. † indicates p < 0.05 for interaction by two-way ANOVA with repeated measures. ∗ indicates p < 0.05 by two-tailed Student’s unpaired t-test (or Mann-Whitney for H). Data are mean +/− SEM.
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K ir 5.1 DN animals fail to mount an adequate physiological response to dietary K + depletion (A–C) K ir 5.1 DN mice had increased urine K + concentrations, B) urine K + excretions, and C) cumulative urine K + losses compared with K ir 5.1 WT controls on a K + -free diet. (D and E) Urine Na + D) concentrations and E) excretions did not differ between genotypes. (F–J) K ir 5.1 DN mice had increased urine Na + -to-K + ratios under K + -free conditions. After consuming a K + -free diet for four days, K ir 5.1 DN mice had G) unchanged blood Na + , H) reduced blood K + , I) unchanged blood Cl − , and J) reduced blood HCO 3 − relative to K ir 5.1 WT controls. Note, K + values in the K ir 5.1 DN group were at the lower limit of detection. A nonparametric analysis was performed for K + , given the lack of normality. (K) Urine K + excretion in two groups of K ir 5.1 DN animals. One group was treated with a K + -free diet alone, while the other group was additionally treated with the ENaC inhibitor, triamterene. (L) Kidney Western blot data after the consumption of a K + -free diet for four days. Compared with K ir 5.1 WT controls, K ir 5.1 DN mice had reduced abundances of NBCe1 and <t>NHE3</t> and increased NKCC2 abundance. Abundances of pNCC and total NCC trended toward being reduced, but did not achieve the threshold for statistical significance. Ponceau shown for pNCC and NHE3. Ponceaus for NBCe1, NKCC2, and tNCC included as . N = 5 and 7 for A-F. N = 10 and 8 for G-J. N = 4 per group for K. N = 5 per group for L. # indicates p < 0.05 by two-way ANOVA with repeated measures followed by Sidak’s post-hoc test. † indicates p < 0.05 for interaction by two-way ANOVA with repeated measures. ∗ indicates p < 0.05 by two-tailed Student’s unpaired t-test (or Mann-Whitney for H). Data are mean +/− SEM.
Nhe3 Ab, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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K ir 5.1 DN animals fail to mount an adequate physiological response to dietary K + depletion (A–C) K ir 5.1 DN mice had increased urine K + concentrations, B) urine K + excretions, and C) cumulative urine K + losses compared with K ir 5.1 WT controls on a K + -free diet. (D and E) Urine Na + D) concentrations and E) excretions did not differ between genotypes. (F–J) K ir 5.1 DN mice had increased urine Na + -to-K + ratios under K + -free conditions. After consuming a K + -free diet for four days, K ir 5.1 DN mice had G) unchanged blood Na + , H) reduced blood K + , I) unchanged blood Cl − , and J) reduced blood HCO 3 − relative to K ir 5.1 WT controls. Note, K + values in the K ir 5.1 DN group were at the lower limit of detection. A nonparametric analysis was performed for K + , given the lack of normality. (K) Urine K + excretion in two groups of K ir 5.1 DN animals. One group was treated with a K + -free diet alone, while the other group was additionally treated with the ENaC inhibitor, triamterene. (L) Kidney Western blot data after the consumption of a K + -free diet for four days. Compared with K ir 5.1 WT controls, K ir 5.1 DN mice had reduced abundances of NBCe1 and NHE3 and increased NKCC2 abundance. Abundances of pNCC and total NCC trended toward being reduced, but did not achieve the threshold for statistical significance. Ponceau shown for pNCC and NHE3. Ponceaus for NBCe1, NKCC2, and tNCC included as . N = 5 and 7 for A-F. N = 10 and 8 for G-J. N = 4 per group for K. N = 5 per group for L. # indicates p < 0.05 by two-way ANOVA with repeated measures followed by Sidak’s post-hoc test. † indicates p < 0.05 for interaction by two-way ANOVA with repeated measures. ∗ indicates p < 0.05 by two-tailed Student’s unpaired t-test (or Mann-Whitney for H). Data are mean +/− SEM.

Journal: iScience

Article Title: K ir 5.1-modulated potassium flux stimulates an anabolic kidney phenotype

doi: 10.1016/j.isci.2025.113601

Figure Lengend Snippet: K ir 5.1 DN animals fail to mount an adequate physiological response to dietary K + depletion (A–C) K ir 5.1 DN mice had increased urine K + concentrations, B) urine K + excretions, and C) cumulative urine K + losses compared with K ir 5.1 WT controls on a K + -free diet. (D and E) Urine Na + D) concentrations and E) excretions did not differ between genotypes. (F–J) K ir 5.1 DN mice had increased urine Na + -to-K + ratios under K + -free conditions. After consuming a K + -free diet for four days, K ir 5.1 DN mice had G) unchanged blood Na + , H) reduced blood K + , I) unchanged blood Cl − , and J) reduced blood HCO 3 − relative to K ir 5.1 WT controls. Note, K + values in the K ir 5.1 DN group were at the lower limit of detection. A nonparametric analysis was performed for K + , given the lack of normality. (K) Urine K + excretion in two groups of K ir 5.1 DN animals. One group was treated with a K + -free diet alone, while the other group was additionally treated with the ENaC inhibitor, triamterene. (L) Kidney Western blot data after the consumption of a K + -free diet for four days. Compared with K ir 5.1 WT controls, K ir 5.1 DN mice had reduced abundances of NBCe1 and NHE3 and increased NKCC2 abundance. Abundances of pNCC and total NCC trended toward being reduced, but did not achieve the threshold for statistical significance. Ponceau shown for pNCC and NHE3. Ponceaus for NBCe1, NKCC2, and tNCC included as . N = 5 and 7 for A-F. N = 10 and 8 for G-J. N = 4 per group for K. N = 5 per group for L. # indicates p < 0.05 by two-way ANOVA with repeated measures followed by Sidak’s post-hoc test. † indicates p < 0.05 for interaction by two-way ANOVA with repeated measures. ∗ indicates p < 0.05 by two-tailed Student’s unpaired t-test (or Mann-Whitney for H). Data are mean +/− SEM.

Article Snippet: NHE3 , Stressmarq , SPC-400.

Techniques: Western Blot, Two Tailed Test, MANN-WHITNEY